ICSI certification has two parts: proficiency and theory. References below are for the theoretical part of examination.

History:

Uehara T, Yanagimachi R. Microsurgical injection of spermatozoa into hamster eggs with subsequent transformation of sperm nuclei into male pronuclei. Biol Reprod. 1976 Nov;15(4):467-70

Lanzendorf SE, Maloney MK, Veeck LL, Slusser J, Hodgen GD, Rosenwaks Z.
A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril. 1988 May;49(5):835-42.

Fishel S, Jackson P, Antinori S, Johnson J, Grossi S, Versaci C. Subzonal insemination for the alleviation of infertility. Fertil Steril. 1990 Nov;54(5):828-35.

Cohen J, Talansky BE, Malter H, Alikani M, Adler A, Reing A, Berkeley A, Graf M, Davis O, Liu H, et al. Hum Reprod. 1991 Jan;6(1):118-23. Microsurgical fertilization and teratozoospermia.

The first ICSI pregnancy

Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Lancet. 1992 Jul 4;340(8810):17-8.

ICSI technique modifications:

Nagy ZP, Oliveira SA, Abdelmassih V, Abdelmassih R Novel use of laser to assist ICSI for patients with fragile oocytes: a case report. Reprod Biomed Online. 2002 Jan-Feb;4(1):27-31.

ICSI with non-ejaculated spermatozoa

Devroey P, Liu J, Nagy Z, Tournaye H, Silber SJ, Van Steirteghem AC. Normal fertilization of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection. Fertil Steril. 1994 Sep;62(3):639-41.

Silber SJ, Nagy ZP, Liu J, Godoy H, Devroey P, Van Steirteghem AC. Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. Hum Reprod. 1994 Sep;9(9):1705-9.

Silber SJ, Johnson L. Are spermatid injections of any clinical value? ROSNI and ROSI revisited. Round spermatid nucleus injection and round spermatid injection. Hum Reprod. 1998 Mar;13(3):509-15.

Crabbé E, Verheyen G, Silber S, Tournaye H, Van de Velde H, Goossens A, Van Steirteghem A. Enzymatic digestion of testicular tissue may rescue the intracytoplasmic sperm injection cycle in some patients with non-obstructive azoospermia.
Hum Reprod. 1998 Oct;13(1O):2791-6.

Rescue ICSI

Nagy ZP, Joris H, Liu J, Staessen C, Devroey P, Van Steirteghem AC.Intracytoplasmic single sperm injection of 1-day-old unfertilized human oocytes.
Hum Reprod. 1993 Dec;8(12):2180-4.

Morton PC, Yoder CS, Tucker MJ, Wright G, Brockman WD, Kort HI.Reinsemination by intracytoplasmic sperm injection of 1-day-old oocytes after complete conventional fertilization failure. Fertil Steril. 1997 Sep;68(3):488-91.

Mechanism of ICSI

Stice SL, Robl JM. Activation of mammalian oocytes by a factor obtained from rabbit sperm. Mol Reprod Dev. 1990 Mar;25(3):272-80.

Tesarik J, Sousa M, Testart J. Human oocyte activation after intracytoplasmic sperm injection. Hum Reprod. 1994 Mar;9(3):511-8.

Dozortsev D, Rybouchkin A, De Sutter P, Qian C, Dhont M. Human oocyte activation following intracytoplasmic injection: the role of the sperm cell. Hum Reprod. 1995 Feb;10(2):403-7.

Dozortsev D, Rybouchkin A, De Sutter P, Dhont M. Sperm plasma membrane damage prior to intracytoplasmic sperm injection: a necessary condition for sperm nucleus decondensation. Hum Reprod. 1995 Nov;10(11):2960-4.

Parrington J, Swann K, Shevchenko VI, Sesay AK, Lai FA. Calcium oscillations in mammalian eggs triggered by a soluble sperm protein. Nature. 1996 Jan 25;379(6563):364-8.

Saunders CM, Larman MG, Parrington J, Cox LJ, Royse J, Blayney LM, Swann K, Lai FA. PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development. Development. 2002 Aug;129(15):3533-44.

ICSI in cases of activation factor deficiency

Rybouchkin AV, Van der Straeten F, Quatacker J, De Sutter P, Dhont M.
Fertilization and pregnancy after assisted oocyte activation and intracytoplasmic sperm injection in a case of round-headed sperm associated with deficient oocyte activation capacity. Fertil Steril. 1997 Dec;68(6):1144-7.

Murase Y, Araki Y, Mizuno S, Kawaguchi C, Naito M, Yoshizawa M, Araki Y.
Pregnancy following chemical activation of oocytes in a couple with repeated failure of fertilization using ICSI: case report. Hum Reprod. 2004 Jul;19(7):1604-7. Epub 2004 Apr 29.

Kyono K, Kumagai S, Nishinaka C, Nakajo Y, Uto H, Toya M, Sugawara J, Araki Y. Birth and follow-up of babies born following ICSI using SrCl2 oocyte activation. Reprod Biomed Online. 2008 Jul;17(1):53-8.

Egashira A, Murakami M, Haigo K, Horiuchi T, Kuramoto T. A successful pregnancy and live birth after intracytoplasmic sperm injection with globozoospermic sperm and electrical oocyte activation. Fertil Steril. 2009 Dec;92(6):2037.e5-9. Epub 2009 Oct 1.

Nasr-Esfahani MH, Deemeh MR, Tavalaee M. Artificial oocyte activation and intracytoplasmic sperm injection. Fertil Steril. 2009 Apr 24.

ICSI with non-ejaculated spermatozoa

Devroey P, Liu J, Nagy Z, Tournaye H, Silber SJ, Van Steirteghem AC. Normal fertilization of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection. Fertil Steril. 1994 Sep;62(3):639-41.

Silber SJ, Nagy ZP, Liu J, Godoy H, Devroey P, Van Steirteghem AC. Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. Hum Reprod. 1994 Sep;9(9):1705-9.

Tournaye H, Liu J, Nagy PZ, Camus M, Goossens A, Silber S, Van Steirteghem AC, Devroey P. Correlation between testicular histology and outcome after intracytoplasmic sperm injection using testicular spermatozoa. Hum Reprod. 1996 Jan;11(1):127-32.

Silber SJ, Johnson L. Are spermatid injections of any clinical value? ROSNI and ROSI revisited. Round spermatid nucleus injection and round spermatid injection. Hum Reprod. 1998 Mar;13(3):509-15.

Tesarik J Oocyte activation after intracytoplasmic injection of mature and immature sperm cells. Hum Reprod. 1998 Apr;13 Suppl 1:117-27.

Ramasamy R, Lin K, Gosden LV, Rosenwaks Z, Palermo GD, Schlegel PN.
High serum FSH levels in men with nonobstructive azoospermia does not affect success of microdissection testicular sperm extraction. Fertil Steril. 2009 Aug;92(2):590-3. Epub 2008 Oct 29.

Spermatozoa-affecting syndromes and ICSI

Bourne H, Richings N, Harari O, Watkins W, Speirs AL, Johnston WI, Baker HW.
The use of intracytoplasmic sperm injection for the treatment of severe and extreme male infertility. Reprod Fertil Dev. 1995;7(2):237-45

von Zumbusch A, Fiedler K, Mayerhofer A, Jessberger B, Ring J, Vogt HJ. Birth of healthy children after intracytoplasmic sperm injection in two couples with male Kartagener's syndrome. Fertil Steril. 1998 Oct;70(4):643-6.

Abu-Musa A, Hannoun A, Khabbaz A, Devroey P. Failure of fertilization after intracytoplasmic sperm injection in a patient with Kartagener's syndrome and totally immotile spermatozoa: case report. Hum Reprod. 1999 Oct;14(10):2517-8
Dam AH, Feenstra I, Westphal JR, Ramos L, van Golde RJ, Kremer JA. Globozoospermia revisited. Hum Reprod Update. 2007 Jan-Feb;13(1):63-75. Epub 2006 Sep 28.

Short tail sperm cells after vasectomy reference needed

ICSI and oocytes dysmorphism

Alikani M, Palermo G, Adler A, Bertoli M, Blake M, Cohen J. Intracytoplasmic sperm injection in dysmorphic human oocytes. Zygote. 1995 Nov;3(4):283-8.
 

Please address all questions about the references to the education committee at education@embcol.org.